玉米S组细胞质雄性不育线粒体R区序列与多型性分析

玉米Ｓ组细胞难性不育（ＣＭＳ）可能与线粒体基因组中的Ｒ区域有关。对不同核背景下唐徐、双２种Ｓ胞质的线粒体ＤＮＡ以Ｒ区特异探针的Ｓｏｕｔｈｅｒｎ分析发现均有６．７ｋｂ、４．５ｋｂ、１．８ｋｂ的３条谱带,分别对应于２种位于线粒体基因组中间的类型和１个线性末端,并且核背景对这３种不同形式的Ｒ区域的量有影响。对Ｍｏ１７和７７核背景下Ｎ、Ｔ、Ｃ４种胞质１７种材料的玉米线粒体基因组中Ｒ区的Ｓｏｕｔｈｅｒｎ分析

Analysis of Sequence and Polymorphism of R in S-type CMS Maize

The S-type CMS of maize was reported to be caused by a DNA region designated R in mitochondrial genomes. Through PCR using artificial primers, 1kb DNA fragments were obtained from mitochondrial DNA of Tangxu and WB cytoplasm.The DNA sequences are almost the same as the sequence in R region of CMS-S. Using the DNA fragment from Tangxu cytoplasm as probe to scan the Mo17 CMS-J gene library, several positive clones appeared. The clone B39 contains a 6.7kb BamHI fragment with R region. Nucleotide sequence analysis found two orfs including the chimeric orf77. Seventeen lines of maize under Mo17 and 77 nuclear background including N, T, C, S four types of cytoplasm were used for RFLP. Three types of R were discovered in S-type CMS, but not in C group. The R in N group was different from that in S group. T group had only part of R region. The Southern blot of Tangx and Shuang cytoplasm in R regions showed different ratio of R in different nuclear backgrounds. The heterogeneity of R region suggests that the organization and rearrangement of R controlled by the nuclear background. R region seems to be involved in the mechanism of S group CMS. Varieties of structures of R, the ratio of three R types, different DNA sequences in R region of different CMS mightbe concerned with instability of S-type CMS.