New arrays of cytoplasmic microtubules in the fission yeastSchizosaccharomyces pombe |
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| Authors: | A. Pichová S. D. Kohlwein M. Yamamoto |
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| Affiliation: | (1) Department of Biophysics and Biochemistry, School of Science, University of Tokyo, P.O. Hongo, 113 Tokyo, Japan;(2) Laboratory of Cell Reproduction, Institute of Microbiology, Czech Academy of Sciences, Prague;(3) Institute of Biochemistry, Technical University of Graz, Graz |
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| Abstract: | Summary New arrays of microtubules in the fission yeastSchizosaccharomyces pombe, which distribute in the cell in a cell cycle-dependent manner, were characterized using conventional and confocal laser scanning immunofluorescence microscopy. During the interphase and prophase, we observed abundant cytoplasmic microtubules between cell poles, a peripheral network of randomly and helically distributed cortical microtubules, and perinuclear microtubules surrounding the nucleus. At the anaphase and telophase, an equatorial ring containing tubulin was visualized. This ring colocalized with an actin contractile ring, suggesting that they may control the plane of cell division cooperatively.Abbreviations MT(s) microtubule(s) - cMT(s) cytoplasmic microtubule(s) - CLSM confocal laser scanning microscopy - DAPI 4 ,6-diamidino-2-phenylindole |
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| Keywords: | Cell division cycle Confocal laser scanning microscopy F-Actin Microtubules Schizosaccharomyces pombe Tubulin ring |
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