两种甲型肝炎病毒抗原快速检测方法的建立

目的建立两种甲型肝炎病毒抗原(HAV-Ag)检测试剂盒,并对其检测效果进行评价。方法生物素标记甲型肝炎病毒抗体(HAV-Ab)与辣根过氧化物酶标记亲和素联合应用建立甲型肝炎病毒抗原BA-ELISA检测法;同时使用辣根过氧化物酶标记HAV-Ab作放大系统建立双抗体夹心甲型肝炎病毒抗原ELISA检测试剂,对比两种检测方法的特异性、灵敏度及实际应用效果。结果用生物素标记甲型肝炎病毒抗体-辣根过氧化物酶标记亲和素作放大系统建立的甲型肝炎病毒抗原BA-ELISA检测法,较双抗体夹心ELISA检测方法灵敏度高1～2个稀释度;两种检测法均对10余种病毒无交叉,P/N值BA-ELISA检测法较高。结论甲型肝炎病毒抗原BA-ELISA检测法是一种灵敏度高,特异性好,方便快捷的检测方法,可广泛应用于甲型肝炎病毒研究及临床检测中。而甲型肝炎病毒抗原双抗体夹心ELISA检测法,检测灵敏度适中,操作简单,更适用于甲肝疫苗生产检定。

Development of two rapid detection methods for hepatitis A virus

Objective To establish two kinds of rapid detection methods for hepatitis A virus antigen(HAV-Ag)and evaluate the detection effect.Methods Hepatitis A virus antigen BA-ELISA assay was established by biotin-labeled hepatitis A virus antibody(HAV-Ab) in combination with avidin-labeled HRP.Double antibody sandwich method of ELISA assay was established using hepatitis A virus HRP-labeled antibody.Two kinds of detection methods were compared for their specificity,sensitivity and the practical application effect.Results The sensitivity of BA-ELISA detection method is higher 1-2 dilution then that of double antibody sandwich detection method.Two kinds of detection methods have not cross reaction with 10 types of virus.P/N value of BA-ELISA assay is higher.Conclusion The hepatitis A virus antigen BA-ELISA assay is a method that has the advantages of convenient,rapid detection,and can be widely applied to hepatitis A virus research and clinical testing.Double antibody sandwich ELISA assay has moderate detection sensitivity,the operation is simple,and more suitable for hepatitis A vaccine production test.