Improved rate of callus induction from rice anther culture following microscopic staging of microspores in iron alum-haematoxylin |
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| Authors: | H S Gupta D N Borthakur |
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| Institution: | (1) Biotechnology Laboratory, Division of Plant Breeding, ICAR Research Complex for NEH Region, 793004 Laban, Shillong, India;(2) Present address: Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD Nottingham, UK;(3) Present address: North-Eastern Council, 793001 Shillong, India |
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| Abstract: | Summary High frequencies of callusing were obtained in indica rice from the microspores which were staged in acetic acid iron alum-haematoxylin stain prior to culture on G5 medium. Two local varieties,  Khonorullo and Namyi, and two advance pre-release cultures, PK 1-1-3 and PK 12-22, were used in this investigation. All the cultures exhibited a wide adaptation to varying medium; however, the frequency of callusing was highest (45.5%) in PK 1-1-3 followed by PK 12-22 (32.4%) and Khonorullo (31.6%). Cold shock (10 °C) for 11 days enhanced the frequency of callusing by 200% in Khonorullo. |
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| Keywords: | Microspore Anther culture Haploid production Oryza sativa L ssp indica |
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