长期酒精摄入对雄性大鼠生殖系统的损伤

摘要目的：研究长期酒精摄入对雄性大鼠生殖系统的损伤机制。方法：选用8 周龄的SD 大鼠，进行随机分组：对照组（5％蔗糖，口服）；酒精组（4g/kg，口服）。连续12周后，分别取附睾考察精子数目、活力；取血清检测睾酮和促黄体生产素（LH）含量；计算睾丸- 体重比，并检测睾丸中丙二醛（MDA）、谷胱甘肽（GSH）含量以及谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性；同时检测凋亡相关蛋白bax，bcl-2 以及caspase-3 前体和剪切体的蛋白表达。结果：酒精组12 周后，大鼠的睾丸- 体重比明显降低（P<0.05），精子数目减少（P<0.01），精子活力下降（P<0.01）；血清中睾酮含量下降（P<0.05），LH 含量增加（P<0.05）；睾丸中MDA 含量增加（P<0.01），GSH 含量降低（P<0.05），GPx 和SOD活性下降（P<0.01）；凋亡相关蛋白bax 表达增加（P<0.05），caspase-3 剪切体与前体的比值增加（P<0.01）。结论：长期摄入酒精引起的大鼠睾丸内氧化应激水平的增加是其导致其生殖系统损伤的重要因素之一。

Chronic Alcohol Intake Contributes to the Injury in ReproductiveSystemof Male Rats

ABSTRACT Objective:To investigate the mechanism of chronic alcohol intake contributed to the injury in reproductive system ofmale rats.Methods: Male SDrats, aged 8 weeks old, were randomly divided into two groups and treated as follows: (i) control (5%sucrose,orally) daily for 12 weeks, (ii) ethanol (4 g/kg, orally) daily for 12 weeks. 12 weeks later, epididymises were removed and analyzedfor sperm number and motility. Serum was isolated and analyzed for concentration of testosterone and LH. Testis were removed andcontent of MDA and GSH, activity of GPx and SOD, expression of bax, bcl-2, caspase-3 and cleaved caspase-3 were analyzed.Results:12 weeks later, the ratio of testicular weight and body weight decreased in ethanol-treated group (P<0.05). The number and motility ofspermdecreased (P<0.01), and the concentration of testosterone and LHin serumdecreased (P<0.05) and increased (P<0.05), respectively.The content of MDA (P<0.01) and GSH (P<0.05) increased. The activity of GSH and GPx decreased (P<0.01). The expression of bax increased(P<0.05) and the ratio of cleaved caspase-3 and caspase-3 elevated (P<0.01). Conclusion:The augmentation of oxidative stress contributesto the ethanol-induced reproductive injury in male rats.