Transcobalamin II--cobalamin binding sites are present on rabbit germ cells. |
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| Authors: | E Boukhzer A Ennya F Felden A Gérard E Nexo J P Nicolas H Gérard J L Guéant |
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| Institution: | Laboratoire de Biochimie Nutritionnelle, INSERM U 308, Faculté de Médecine, Vandoeuvre-les-Nancy, France. |
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| Abstract: | Specific binding sites for rabbit transcobalamin II have been found on isolated adult rabbit germ cells. Scatchard analysis revealed a single class of binding sites for 57Co]cyanocobalamin-transcobalamin II with an association constant (Ka) of 1.3 x 10(10) M-1 and 700 sites per cell. Binding was reversible, saturable and calcium dependent. Electron microscope radioautography following incubation with iodinated transcobalamin II at 4 degrees C led to a detectable labeling mainly restricted to the plasma membrane. |
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