Chromosome location of the genes for 28S, 18S and 5S ribosomal RNA in Triturus marmoratus (Amphibia Urodela) |
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Authors: | Dr. Giuseppina Barsacchi Pilone Dr. Irma Nardi Dr. Renata Batistoni Francesca Andronico Elena Beccari |
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Affiliation: | 1. Institute of Histology and Embryology, University of Pisa, Italy 2. Laboratory of Molecular Embryology CNR, Arco Felice, Napoli 3. Istituto di Zoologia e Anatomia Comparata, via A. Volta, 4, 66100, Pisa, Italy
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Abstract: | The localization of the 28S, 18S and 5S rRNA genes in the mitotic chromosomes, and of the 5S rRNA genes in the lampbrush chromosomes of Triturus marmoratus has been studied by RNA/DNA in situ hybridization. The 28S and 18S genes are located in a subterminal position, and the 5S genes in an intermediate position, on the long arm of mitotic chromosome X. In situ hybridization on lampbrush chromosomes has shown that the 5S genes are located at or near a dense matrix loop landmark. The cytogenetic implications of these findings are briefly discussed. |
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