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Use of an industrial grade medium and medium enhancing effects on high cell density CO fermentation by Eubacterium limosum KIST612 |
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| Authors: | In Seop Chang Daehee Kim Byung Hong Kim Robert W. Lovitt |
| Affiliation: | (1) Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju, 500-712, Korea;(2) Water Environment & Remediation Research Centre, Korea Institute of Science and Technology, 39-1, Hawolgok-dong, Sungpook-ku, Seoul, 136-791, Korea;(3) Multidisciplinary Nanotechnology Centre, School of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP, UK |
| Abstract: | Studies were made on the composition of the growth medium to increase the cell concentration in a cell-recycled continuous culture (Eubacterium limosum KIST612) with carbon monoxide as a sole energy source using phosphate-buffered basal medium (PBBM) and modified PBBM. One of major limiting factors in PBBM might be nitrogen during the high cell density culture. This limitation could be overcome by increasing of inorganic nitrogen or yeast extract concentration in the medium. Anaerobic digester fluid, which could replace the organic nitrogen in PBBM, was used to develop an industrial grade medium for conversion of CO to multi-carbon compound. |
| Keywords: | Carbon monoxide Eubacterium limosum Industrial grade medium |
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