益气补肾方药对肾虚小鼠细胞因子IL-1、IL-2及IL-12基因表达的影响

目的　探讨益气补肾中药对醋酸可的松致肾虚小鼠腹腔巨噬细胞IL 1及脾脏细胞因子IL 2、IL 12活性及脾脏IL 1、IL 2、IL 12mRNA表达的影响。方法　用醋酸可的松制备肾虚动物模型 ,经益气补肾方药灌胃给药 6周后 ,生物学方法观察腹腔巨噬细胞IL 1、脾脏淋巴细胞IL 2、IL 12的活性水平 ,RT PCR方法观察脾脏IL 1、IL 2及IL 12mRNA的表达。结果　模型动物IL 1、IL 2及IL 12的活性水平较正常小鼠下降 ,其mRNA表达均受到抑制 ,与正常对照组比差异显著 (P <0 0 5 ) ;经益气补肾方药治疗后 ,三种细胞因子活性水平及其mRNA表达均有不同程度的提高。结论　益气补肾方药可能是改善外源糖皮质激素所致肾虚及由此而导致的免疫衰老较理想的方法

Effect of Tonifying Kidney plus Strengthening Vital Energy on IL-1,IL-2,IL-12 Gene Expression in Mice with Nephrasthenia

Objective To explore the effect of Chinese medicine of tonifying kidney plus strengthening vital energy on production of IL 1,IL 2,IL 12 and their mRNA expression in mice with nephrasthenia. Methods\ Animal model of kidney deficiency was made by corticosterone. The model animal was medicated with tonifying kidney plus strengthening vital energy for 6 weeks, then, the production of IL 1 by peritoneal macrophages, IL 2 and IL 12 by splenetic lymphocytes were assayed with the methods of biological activity analysis and ELISA separately. The mRNA expressions of IL 1,IL 2,IL 12 were examined by RT PCR. Results\ The production of IL 1, IL 2 and IL 12 declined in model animal group, but they were restored in mice treated with Chinese medicine in different degree,and the 3 kinds of mRNA expression were also increased when the mice were given Chinese medicine. Conclusion\ Tonifying kidney plus strengthening vital energy is an ideal method for treating mice with nephrasthenia due to corticosterone, so that it can improve the state of immunosenescence.