Interference of melanin in the susceptibility profile of Sporothrix species to amphotericin B |
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| Affiliation: | 1. Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil;2. Faculdade Meridional Imed, Passo Fundo, Rio Grande do Sul, Brazil;3. Laboratório de Microbiologia Clínica, Centro Universitário Franciscano, Santa Maria, Rio Grande do Sul, Brazil;1. Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, Halsbrücker Straße 34, 09599 Freiberg, Germany;2. Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, Germany;1. Division of Medical Oncology and Haematology, Bras Family Drug Development Program, Princess Margaret Cancer Centre, Toronto, ON, M5G 2M9, Canada;1. Department of Small Animal, Universidade Federal de Santa Maria, Brazil;2. Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil;3. Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil;4. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Brazil;5. Universidade Luteranda do Brasil – ULBRA, Santa Maria, Brazil |
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| Abstract: | ![]()
BackgroundThe presence of melanin in the fungal cell is a major virulence factor of the genus Sporothrix since it protects the fungal cells against the defense systems.AimsThe present study aimed to investigate the interference of melanin in the susceptibility of Sporothrix brasiliensis and Sporothrix schenckii sensu stricto to amphotericin B and itraconazole, drugs recommended as therapy for disseminated and subcutaneous sporotrichosis, respectively.MethodsYeast cells were cultivated in minimal medium with or without l-DOPA in order to induce the production of melanin. Microdilution and killing assay methods were used to determine the antifungal activity against yeast cells with different amounts of melanin.ResultsThe killing assay showed that melanization protected isolates within the S. schenckii complex from amphotericin B, particularly in the lower concentrations tested.ConclusionsStudies combining amphotericin B and inhibitors of melanin are required in order to avoid this effect. |
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| Keywords: | Melanin Resistance Susceptibility Amphotericin B Melanina Resistencia Sensibilidad Anfotericina B |
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