IS<Emphasis Type="Italic">1111</Emphasis> insertion sequences of <Emphasis Type="Italic">Coxiella burnetii</Emphasis>: characterization and use for repetitive element PCR-based differentiation of <Emphasis Type="Italic">Coxiella burnetii</Emphasis> isolates |
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| Authors: | Amy M Denison Herbert A Thompson Robert F Massung |
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| Institution: | (1) Coordinating Center for Infectious Diseases, Division of Viral and Rickettsial Diseases, Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA |
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| Abstract: | Background
Coxiella burnetii contains the IS1111 transposase which is present 20 times in the Nine Mile phase I (9Mi/I) genome. A single PCR primer that binds to each IS
element, and primers specific to a region ~500-bp upstream of each of the 20 IS1111 elements were designed. The amplified products were characterized and used to develop a repetitive element PCR genotyping
method. |
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