Isolation of mesenchymal stem cells from equine umbilical cord blood |
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Authors: | Thomas G Koch Tammy Heerkens Preben D Thomsen Dean H Betts |
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Institution: | 1.Department of Biomedical Sciences,University of Guelph,Guelph,Canada;2.Department of Basic Animal and Veterinary Sciences,University of Copenhagen,Frederiksberg C,Denmark |
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Abstract: | Background There are no published studies on stem cells from equine cord blood although commercial storage of equine cord blood for future
autologous stem cell transplantations is available. Mesenchymal stem cells (MSC) have been isolated from fresh umbilical cord
blood of humans collected non-invasively at the time of birth and from sheep cord blood collected invasively by a surgical
intrauterine approach. Mesenchymal stem cells isolation percentage from frozen-thawed human cord blood is low and the future
isolation percentage of MSCs from cryopreserved equine cord blood is therefore expectedly low. The hypothesis of this study
was that equine MSCs could be isolated from fresh whole equine cord blood. |
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