Effects of various fluorochromes and competition between labeled oligonucleotides on their hybridization to oligonucleotides immobilized on biological microchips |
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| Authors: | E. Yu. Kotova E. Ya. Kreindlin V. E. Barsky A. D. Mirzabekov |
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| Affiliation: | (1) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117989 Moscow, Russia;(2) Biochip Technology Center, 60439 Argonne, IL, USA |
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| Abstract: | Some technical tips are described on how to improve the discrimination between perfect and imperfect duplexes formed by hybridization
of fluorescently labeled oligonucleotides to biological microchips. Model experiments were performed to assess the precision
of the method. Effects of labeling on the efficiency of hybridization and some properties of competitive hybridization were
studied using short synthetic oligonucleotides and three most popular fluorochromes as examples. |
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| Keywords: | biological microchips hybridization hybridization efficiency fluorescently labeled oligonucleotides |
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