FT-IR study of heterologous protein expression in recombinant Escherichia coli strains |
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| Authors: | Ami Diletta Bonecchi Loredana Calì Simona Orsini Gaetano Tonon Giancarlo Doglia Silvia Maria |
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| Affiliation: | INFM UdR Milano-Bicocca, Department of Biotechnologies and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy. |
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| Abstract: | Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation. |
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