Controlled-Heat Fixation of Smears and Thin Tissue Slices in Liquid Mercury |
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Authors: | C. H. Barnett E. T. Cusick R. A. Stockwell |
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Affiliation: | a Department of Anatomy, University of Tasmania, Hobart, Tasmaniab Department of Pathology, Royal Hobart Hospital, Hobart, Tasmaniac Department of Anatomy, St. Thomas's Hospital Medical School, London, SEI, England |
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Abstract: | Fresh tissue slices 2 mm thick and blood films have been fixed by immersion in mercury maintained at 55 C. Fixation is effected in 1.5 min without an excessive temperature rise—the main disadvantage of flaming or hot air used for dry heat fixation. For haematology, in particular, the method is convenient to use as a standard procedure for a fixation that is compatible with many histochemical and most of the usual histological techniques. |
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