LPS诱导海马星形胶质细胞活化与活化的星形胶质细胞对海马神经元的影响

探讨脂多糖(Lipopolysaccharide,LPS)对长时间存活大鼠海马内星形胶质细胞的反应以及对神经元的影响。方法:本实验用10只健康成年雄性SD大鼠,海马CA3区注射LPS 10μ1．7和14d后,尼氏染色观察神经元的变化,免疫组织化学染色结合图像分析方法观察海马CA3区注射部位胶质纤维酸性蛋白(glial fibrillary acidic protein GFAP)、的表达变化。结果:脂多糖可促进海马星形胶质细胞的活化,但并不能引起海马区神经元的损伤。结论:星形胶质细胞在脑损伤后的脑内炎症反应起了一定的作用,但并不能引起神经元的损伤。

The effects of reactive astrocyte induced by LPS on neuron in the hippocampus

To observe the effects of ipopolysaccharide(LPS)-induced astrocyte and neuron in the hippocampus in order to discuss the effectS of astrocyte in the neuroinflammation in the hippocampus. Methods: In the present study, Thirty healthy male Sprague-Dawley(SD)rats were injected with LPS intrahippocampally.After 7and14 d,the rats were sacrificed for GFAP,immunohistochemistry staining and nissle staining.Results:The results showed that,compared with saline- control group,injection of LPS induced significant expression of activation of astrocytes,but can't induced the neuron death in the hippocampus.Conclusions:These findings indicate that the reactive astrocytes have some effects on LPS-induced neuroinflammation,but the reactive astrocytes can't induce the neuron death.