Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors |
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| Authors: | Laurie Burnett Maryanne Arnoldo Stephen Yarrow Bin Huang |
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| Institution: | (1) Pioneer Hi-Bred Production Ltd, 12111 Mississauga Road, L7G 4S7 Georgetown, Ontario, Canada |
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| Abstract: | The presence of benzyladenine or naphthaleneacetic acid in seed germination medium markedly enhanced subsequent shoot regeneration from the base of the excised cotyledon explants of Brassica rapa cv. Horizon. Cotyledon explants from younger seedlings (3 or 4-day old) produced more shoots than those from older seedlings. Addition of the ethylene inhibitor aminoethoxyvinylglycine (1.0  M) to the regeneration medium improved shoot regeneration three fold.Abbreviations AVG
aminoethoxyvinylglycine
- BA
benzyladenine
- MGBG
methylglyoxal-bisguanylhydrazone
- MSBN
ms (murashige & skoog 1962) medium supplemented with 4.4 m BA & 5.4 m NAA, 2% sucrose
- NAA
naphthaleneacetic acid |
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| Keywords: | aminoethoxyvinylglycine benzyladenine methylglyoxal-bis-guanylhydrazone naphthalencacetic acid silver nitrate |
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