Volumetric behavior of the molten globule state of canine milk lysozyme |
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| Authors: | Watanabe Masahiro Kobashigawa Yoshihiro Aizawa Tomoyasu Demura Makoto Nitta Katsutoshi |
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| Affiliation: | Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan. |
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| Abstract: | The effect of pressure on the unfolding of the molten globule (MG) state of canine milk lysozyme (CML) was examined using ultraviolet spectroscopy. The volume changes of the MG-unfolded-state transition were observed at pH 2.0 and around 20 to 60 degrees C, but no volume change has been found for bovine alpha-lactalbumin, which is homologous to CML. Our results suggest that the MG state of CML possesses a tightly packed hydrophobic core. |
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