A comparison of Thai silk fibroin-based and chitosan-based materials on in vitro biocompatibility for bone substitutes |
| |
Authors: | Nuttapon Vachiraroj Juthamas Ratanavaraporn Siriporn Damrongsakkul Rath Pichyangkura Tanom Banaprasert Sorada Kanokpanont |
| |
Institution: | 1. Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand;2. Department of Biochemistry, Faculty of Science, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand;3. Department of Otolaryngology, Faculty of Medicine, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand |
| |
Abstract: | The novel hybrid scaffolds fabricated from silk fibroin, gelatin, low deacetylation degree chitosan and hydroxyapatite were investigated for their in vitro biocompatibility and osteoconductivity to mouse pre-osteoblast cell line (MC3T3-E1) and rat bone marrow-derived stem cells (MSC). We found that gelatin-conjugated silk fibroin films and scaffolds dominantly promoted cell adhesion and proliferation. Film and scaffold prepared from gelatin-conjugated silk fibroin with hydroxyapatite grown crystals effectively enhanced osteogenic differentiation of both cell types, as evaluated by alkaline phosphatase activity and calcium content. However the blend of hydroxyapatite/low deacetylation degree chitosan hybrid materials did not support cell growth. Furthermore, the blended hydroxyapatite in the bulk scaffold was found to be less effective for osteogenic differentiation than the scaffold with hydroxyapatite grown crystals. The comparative study between MC3T3-E1 and MSC showed that both cell types had similar trend of proliferation and osteogenic differentiation on the same material. Also, higher proliferative rate of MC3T3-E1 than MSC was observed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|