Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation |
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Authors: | Eunyoung Jeon Soojin Lee Donghyun Kim Hyunsik Yoon Minkyu Oh Chulhwan Park Jinwon Lee |
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Affiliation: | 1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea;2. Department of Biotechnology, Inha University, Inchun 402-751, Republic of Korea;3. Department of Chemical and Biomolecular Engineering, Korea University, Seoul 136-701, Republic of Korea;4. Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Republic of Korea |
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Abstract: | The main goal of this research was to achieve a more efficient production of 1,2-propanediol (1,2-PD) using mutated Saccharomyces cerevisiae. 1,2-PD cannot be produced by wild type S. cerevisiae. To develop a S. cerevisiae mutant that could produce 1,2-PD, the mgs gene of E. coli-K12 MG1655 and the dhaD gene of Citrobacter freundii were inserted into yeast expression vectors such as pESC-URA and pESC-TRP and transformed into the wild type of S. cerevisiae. As a result, the batch fermentation of S. cerevisiae YPH500, harboring an mgs gene inserted pJES27 vector, resulted in a yield of 0.17 g/L. On the other hand, the methylglyoxal synthase of the recombinant S. cerevisiae YPH500, harboring a dhaD gene inserted pJES29 vector, was inactive and produced no detectable amount of 1,2-PD. Therefore, in order to achieve a maximum yield of 1,2-PD, we selected the pESC-TRP vector that is able to co-express the dhaD gene with the pJES27 vector. By inserting the dhaD gene into the pESC-TRP vector, the pJES30 vector was constructed. The maximal yield of 1,2-PD achieved in a 1% galactose batch fermentation by pJES27 and pJES30 harboring S. cerevisiae was 0.45 g/L. |
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