Comparative structural analyses of purified glycogen particles from rat liver,human skeletal muscle and commercial preparations |
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| Authors: | Je-Hoon Ryu Jace Drain Jung Hwan Kim Sean McGee Angus Gray-Weale Lynne Waddington Glendon J Parker Mark Hargreaves Sang-Ho Yoo David Stapleton |
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| Institution: | 1. Department of Food Science and Technology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea;2. School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia;3. School of Medicine, Deakin University, Geelong, Victoria, Australia;4. School of Chemistry, Monash University, Clayton, Victoria, Australia;5. CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria, Australia;6. University of Utah, Salt Lake City, UT, USA;7. Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia;8. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3010, Australia |
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| Abstract: | Glycogen is a cellular energy store that is crucial for whole body energy metabolism, metabolic regulation and exercise performance. To understand glycogen structure we have purified glycogen particles from rat liver and human skeletal muscle tissues and compared their biophysical properties with those found in commercial glycogen preparations. Ultrastructural analysis of commercial liver glycogens fails to reveal the classical α-rosette structure but small irregularly shaped particles. In contrast, commercial slipper limpet glycogen consists of β-particles with similar branching and chain lengths to purified rat liver glycogen together with a tendency to form small α-particles, and suggest it should be used as a source of glycogen for all future studies requiring a substitute for mammalian liver glycogen. |
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