FISH荧光原位杂交技术在污水生物脱氮研究中的应用

简要介绍了荧光原位杂交（FISH）的基本原理，着重讨论近年来该技术在污水生物脱氮研究中的应用现状和特点。研究表明：FISH技术能够准确地表现污水处理反应器中脱氮菌群落的类型和结构形态。但在关于SRT、DO、C／N比等工艺参数的变化对脱氮反应器中微生物类型、数量和结构的影响等方面的研究还有待深入。FISH技术与PCR—DGGE和16SrRNA／rDNA序列分析等技术相结合是对污水处理构筑物中生物脱氮群落深入研究的发展方向。

Application of Fluorescence in Situ Hybridization in Research on Biological Removal of Nitrogen from Wastewater

After brief introduction of FISH (fluorescence in situ hybridization),it was discussed of the FISH's application status in research on biological nitrogen removal in recent years. Base on the FISH technology,Characterization of the community in biological reactor could be showed exactly,but more research should be carried out for the study on the effluence on microbial community composition,which was caused by changing operating parameter of biological reactors on the microbial community composition,such as SRT,DO and C/N ratio.The combination of FISH and other methods such as PCR-DGGE and 16S rRNA/rDNA will led to the identification of the microbial community which response for the nitrogen-removal in wastewater treatment plant.