Ethanol production from biomass by repetitive solid-state fed-batch fermentation with continuous recovery of ethanol |
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Authors: | Churairat Moukamnerd Masahiro Kino-oka Minetaka Sugiyama Yoshinobu Kaneko Chuenchit Boonchird Satoshi Harashima Hideo Noda Kazuaki Ninomiya Suteaki Shioya Yoshio Katakura |
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Institution: | (1) Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;(2) Faculty of Science, Department of Biotechnology, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand;(3) Kansai Chemical Engineering Co., Ltd., 2-9-7 Minami-nanamatsu-cho, Amagasaki 660-0053, Japan;(4) Institute of International Environment Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;(5) Department of Applied Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan |
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Abstract: | To save cost and input energy for bioethanol production, a consolidated continuous solid-state fermentation system composed
of a rotating drum reactor, a humidifier, and a condenser was developed. Biomass, saccharifying enzymes, yeast, and a minimum
amount of water are introduced into the system. Ethanol produced by simultaneous saccharification and fermentation is continuously
recovered as vapor from the headspace of the reactor, while the humidifier compensates for the water loss. From raw corn starch
as a biomass model, 95 ± 3, 226 ± 9, 458 ± 26, and 509 ± 64 g l−1 of ethanol solutions were recovered continuously when the ethanol content in reactor was controlled at 10–20, 30–50, 50–70
and 75–85 g kg-mixture−1, respectively. The residue showed a lesser volume and higher solid content than that obtained by conventional liquid fermentation.
The cost and energy for intensive waste water treatment are decreased, and the continuous fermentation enabled the sustainability
of enzyme activity and yeast in the system. |
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