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Polygalacturonase activity and location in arbuscular mycorrhizal roots of Allium porrum L.
Authors:Renato Peretto  Paola Bonfante  Vittorio Bettini  Francesco Favaron  Paolo Alghisi
Affiliation:(1) Dipartimento di Biologia, Vegetale dell'Università, Viale Mattioli, 25, I-10125 Turin, Italy;(2) Centro di Studio Sulla Micologia del Terreno, CNR, Viale Mattioli, 25, I-10125 Turin, Italy;(3) Istituto di Patologia Vegetale, Università di Padova, Via Gradenigo, 6, I-35131 Padua, Italy
Abstract:Polygalacturonase activity and location were analysed in leek roots (Allium porrum L.) colonized by Glomus versiforme (Karst.) Berch, an arbuscular mycorrhizal (AM) fungus. Polygalacturonase activity in mycorrhizal roots did not differ quantitatively from that found in nonmycorrhizal roots on all of the four harvesting dates. Fractionation of mycorrhizal root extracts by ion-exchange chromatography showed that expression of polygalacturonase was specific to the mutualistic association. Immunofluorescence and immunogold experiments were carried out to locate the polygalacturonase in mycorrhizal roots using a polyclonal antibody raised against a Fusarium moniliforme endopolygalacturonase. Immunolabelling was observed all over the arbuscules (intracellular fungal structures) but particularly at the interface between the arbuscule and the plant membrane. Since pectins are located in this area, we suggest that polygalacturonase produced during the symbiosis could play a role in plant pectin degradation.
Keywords:Allium porrum  Arbuscular mycorrhiza  Pectin  Polygalacturonase  Immunolocation
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