Analysis of flumequine enantiomers in rat plasma by UFLC‐ESI‐MS/MS |
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Authors: | Zhao–Hong Hu Yang–Guang Wang Huo–Xi Jin Xiao–Kun Ouyang Wei–Jian Wu |
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Institution: | School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China |
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Abstract: | In this study the analysis and confirmation of flumequine enantiomers in rat plasma by ultra‐fast liquid chromatography coupled with electron spray ionization mass spectrometry (using propranolol as an internal standard IS]) was developed and validated. Plasma samples were prepared by liquid–liquid extraction using methyl tert‐butyl ether as the extraction solvent. Direct resolution of the R‐ and S‐isomers was performed on a CHIRALCEL OJ‐RH column (4.6 × 150 mm, 5 μm) using acetonitrile / 0.1% formic acid / 1 mM ammonium acetate as the mobile phase. Detection was operated by electron spray ionization in the selected ion monitoring and positive ion mode. The target ions at m/z 262.1 and m/z 260.1 were selected for the quantification of the enantiomers and IS, respectively. The linear range was 0.5–500 ng/mL. The precisions (coefficient of variation, CV%) and recoveries were 1.43–8.68 and 94.24–106.76%, respectively. The lowest quantitation limit for both enantiomers is 0.5 ng/mL, which is sensitive enough to be applied to sample analysis in other related studies. |
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Keywords: | chiral separation flumequine LC– MS/MS rat plasma |
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