| NRSE/RE-1 dsRNA诱导小鼠胚胎干细胞分化为NSE阳性的神经元样细胞 |
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| 引用本文： | 李宏图,顾文佳,孟凡彪,马兰兰,庞希宁. NRSE/RE-1 dsRNA诱导小鼠胚胎干细胞分化为NSE阳性的神经元样细胞[J]. 生命科学研究, 2009, 13(6): 477-481 |
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| 作者姓名： | 李宏图 顾文佳 孟凡彪 马兰兰 庞希宁 |
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| 作者单位： | 1. 中国医科大学发育生物学教研室,卫生部细胞生物学重点实验室,中国辽宁,沈阳,110001;辽宁省计划生育科学研究院辽宁省生殖医学重点实验室,中国辽宁,沈阳,110031
2. 中国医科大学发育生物学教研室,卫生部细胞生物学重点实验室,中国辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省自然科学基金资助项目，辽宁省教育厅资助项目 |
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| 摘 要： | 应用电打孔的方法将化学合成的NRSE/RE-1d sRNA转染小鼠胚胎干细胞,在去除LIF的条件下,直接接种培养,观察其分化情况,并对分化结果进行相关检测.结果显示分化细胞呈明显的神经样改变,免疫荧光显示NSE阳性率为（82.3±8.1）%.说明通过转染NRSE/RE-1d sRNA能有效地诱导小鼠胚胎干细胞向神经元细胞分化.
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| 关 键 词： | 胚胎干细胞 NRSE/RE-1d sRNA 诱导分化 神经元样细胞 |
Effect of NRSE/RE-1 dsRNA in Inducing Differentiation of Mouse Embryonic Stem Cells into Neuron-like Cells |
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| LI Hong-tu,GU Wen-jia,MENG Fan-biao,MA Lan-lan,PANG Xi-ning. Effect of NRSE/RE-1 dsRNA in Inducing Differentiation of Mouse Embryonic Stem Cells into Neuron-like Cells[J]. Life Science Research, 2009, 13(6): 477-481 |
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| Authors: | LI Hong-tu GU Wen-jia MENG Fan-biao MA Lan-lan PANG Xi-ning |
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| Affiliation: | LI Hong-tu1,2,GU Wen-jia1,MENG Fan-biao,MA Lan-lan1,PANG Xi-ning1* (1. Department of Developmental Biology,Chinese Medical University,Key Laboratory of Cell Biology,Ministry of Public Health,Shenyang 110001,Liaoning,China,2. Liaoning Provincial Research Institute of Family Planning,Key Laboratory of Reproduction Health of Liaoning Province,Shenyang 110031,China) |
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| Abstract: | Mouse embryonic stem cells were transfected with NRSE/RE-1 dsRNA through the electroporation and then cultured in medium without LIF.The differentiated cells were observed and detected by related means.Their morphology were obviously neuron-like alteration and(82.3±8.1)%NSE-positive.These results showed that the exogenous NRSE/RE-1dsRNA can efficiently induce ESCs differentiate into neurons in vitro. |
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| Keywords: | NRSE/RE-1dsRNA embryonic stem cells NRSE/RE-1dsRNA neural induction neuron-like cells |
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