GTP and GDP will stimulate platelet cytosolic phospholipase C independently of Ca2+ |
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| Authors: | Y Banno S Nakashima T Tohmatsu Y Nozawa E G Lapetina |
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| Affiliation: | 1. Department of Medicine, University of Otago Christchurch, Christchurch, New Zealand;2. Centre for Kidney Research, The Children’s Hospital at Westmead, Sydney, Australia;3. Sydney School of Public Health, University of Sydney, Sydney, Australia;4. Department of Clinical Pharmacology and Epidemiology, Fondazione Mario Negri Sud, S. Maria Imbaro, Italy;5. Department of Emergency and Organ Transplantation, University of Bari, Bari, Italy;6. Department of Translational Medicine, Division of Nephrology and Transplantation, Amedeo Avogadro University of Eastern Piedmont, Novara, Italy;7. Diaverum Medical Scientific Office, Lund, Sweden;8. Department of Nephrology, Princess Alexandra Hospital, Brisbane, Queensland, Australia;9. Translational Research Institute, Brisbane, Queensland, Australia;1. Division of Nephrology, Department of Medicine, Winthrop University Hospital, Mineola, New York, USA;2. Department of Clinical Health Professions, St. John’s University College of Pharmacy and Health Sciences, Queens, New York, USA;3. Department of Pharmacy, The Zucker Hillside Hospital, Queens, New York, USA;4. Division of Nephrology, Department of Medicine, University of Illinois, Chicago, Illinois, USA;1. Department of Neurology, Weill Cornell Medicine, New York, NY, USA;2. Destroke, Inc., USA;3. Burke Neurological Institute, White Plains, NY, USA;4. Westchester Medical Center, Valhalla, NY, USA |
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| Abstract: | ![]()
The hydrolysis of [3H]phosphatidylinositol 4,5-bisphosphate (PIP2) by cytosolic phospholipase C from human platelets was determined. Cytosolic fractions were prepared from platelets that had or had not been preactivated with thrombin. Thrombin pretreatment did not affect cytosolic phospholipase C activity. In both cytosolic fractions, phospholipase C was activated by GTP and GTP gamma S. This action is observed in the presence of 2 mM EGTA. GDP was as effective as GTP in stimulating cytosolic phospholipase C in the presence of Ca2+ or EGTA. Partially purified phospholipase C obtained from platelet cytosol is activated by GTP, but not by GTP gamma S, in the presence of 2 mM EGTA. However, in the presence of 6 microM Ca2+, both GTP and GTP gamma S stimulated the partially purified phospholipase C. Our present information indicates that GTP and GDP have a direct effect on the cytosolic phospholipase C. |
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