Variable Spontaneous Mutation and Loss of Heterozygosity among Heterozygous Genomes in Yeast |
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| Authors: | Duong T Nguyen Baojun Wu Hongan Long Nan Zhang Caitlyn Patterson Stephen Simpson Krystalynne Morris W Kelley Thomas Michael Lynch Weilong Hao |
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| Institution: | 1. Department of Biological Sciences, Wayne State University, Detroit, MI;2. Institute of Evolution and Marine Biodiversity, KLMME, Ocean University of China, Qingdao, Shandong Province, China;3. Department of Biology, Indiana University, Bloomington, IN;4. University of New Hampshire, Durham, NH;5. Center for Mechanisms of Evolution, The Biodesign Institute, Arizona State University, Tempe, AZ |
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| Abstract: | Mutation and recombination are the primary sources of genetic variation. To better understand the evolution of genetic variation, it is crucial to comprehensively investigate the processes involving mutation accumulation and recombination. In this study, we performed mutation accumulation experiments on four heterozygous diploid yeast species in the Saccharomycodaceae family to determine spontaneous mutation rates, mutation spectra, and losses of heterozygosity (LOH). We observed substantial variation in mutation rates and mutation spectra. We also observed high LOH rates (1.65–11.07×10−6 events per heterozygous site per cell division). Biases in spontaneous mutation and LOH together with selection ultimately shape the variable genome-wide nucleotide landscape in yeast species. |
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| Keywords: | base substitutions mutation rate mutational spectrum GC content diploid genome LOH |
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