Measurement of an intracellular pH rise after fertilization in crab eggs using 31P-NMR |
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| Authors: | M. Hervé M. Goudeau J. M. Neumann J. C. Debouzy H. Goudeau |
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| Affiliation: | (1) Service de Biophysique, CEN Saclay, F-91191 Gif-sur-Yvette Cédex, France;(2) UFR Santé Médecine Biologie Humaine, Université Paris XIII, F-93012 Bobigny, France;(3) UA-CNRS 686, F-29211 Roscoff, France;(4) Station Marine de Roscoff, F-29211 Roscoff, France |
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| Abstract: | The effect of fertilization upon the intracellular pH, pHi, in crab ovulated eggs was examined by 31P-NMR. The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. The detection of the Pi peak was accomplished by Hahn spin-echo experiments in order to cancel the broad signal arising from phosphoproteins which overlaps the Pi signal. The average pHi of the unfertilized unactivated eggs was 6.55 and a rise of 0.12 pH unit occurred after fertilization. |
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| Keywords: | Crab eggs fertilization 31P-NMR intracellular pH 31P transverse relaxation in cells |
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