Enhancing the mitotic index of blastomeres by thymidine synchronization |
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Authors: | O.J. Rottmann S. Arnold |
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Affiliation: | Lehrstuhl für Tierzucht der Technischen Universität München, 8050 Freising Weihenstephan Federal Republic of Germany |
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Abstract: | Mouse blastomeres were synchronized by exposure of the embryo to thymidine and colchicine. The mitosis index increased from 9% metaphases (colchicine synchronization) to 18% (thymidine + colchicine synchronization) per embryo. The in vitro development of embryos was not affected by treatment. This method appears to approach a level where successful sexing of embryos becomes possible. |
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