Molecular characterization and association analysis of <Emphasis Type="Italic">FBXO40</Emphasis> with partial hematological indexes in pig |
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Authors: | Z W Wang X Y Li Z L Tang S L Yang Z Z Ying T Fu B Fan Y L Mu H Ao K Li |
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Institution: | (1) The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China;(2) The Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education of China, Huazhong Agricultural University, Wuhan, 430070, PR China |
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Abstract: | F-box proteins are quite significant ubiquitin-proteasome pathway regulators in eukaryotic cells. FBXO40, a member of this large family, alters its expression pattern in muscle atrophy. Here we isolated most of the verified porcine
FBXO40 coding sequence (CDS) (2258 bp) and assigned it to the porcine chromosome 13q4.1-4.6 by using the INRA-Minnesota porcine
radiation hybrid panel, and we also explored the tissue expression distributions, which is relatively high in longissimus
dorsi muscle, heart, low in kidney, small intestine, brain, hypophysis, lymphonode, thymus, spleen, large intestine, ovary,
stomach, and undetectable in testis, liver, uterus and thyroid gland. Inferring phylogenetic tree was constructed to study
the evolutionary implications. Moreover, a HindII (HincII)-RFLP (A/C) polymorphism in 3′-untranslated region (3′-UTR) of porcine FBXO40 gene was demonstrated by sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. Statistical analysis
result of this polymorphism showed that the allele A was predominant in all detected indigenous breeds, but C in western introduced
commercial breeds. The SNP was further analyzed in our experimental pig population including Tongcheng, Landrace, Large White,
and crossbreds of Large White × (Landrace × Tongcheng) and Landrace × (Large White × Tongcheng). The association analysis
results indicated that the A/C base substitution was associate with some hematological indexes, the hemoglobin concentration
(P < 0.0001), mean corpuscular volume hemoglobin concentration (P = 0.0002) and mean corpuscular volume (P = 0.0138). |
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