Development of a plant regeneration system based on friable embryogenic callus in the ornamental Alstroemeria |
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Authors: | H -S Lin C van der Toorn K J J M Raemakers R G F Visser MJ De Jeu E Jacobsen |
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Institution: | (1) Graduate School of Experimental Plant Sciences, Laboratorium of Plant Breeding, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL |
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Abstract: | Stem segments of seedlings from two Alstroemeria breeding lines, cultured on media supplemented with 4 mg/l 2,4-dichlorophenoxyacetic acid and 0.5–1.0 mg/l 6-benzylaminopurine
(BA), initiated soft callus, which became compact after subculture on a medium with only 0.5 mg/l BA. Friable embryogenic
calli were initiated from compact callus on a medium supplemented with 10 mg/l picloram. Proembryos developed from friable
embryogenic calli via embryos into plants after subculture on medium supplemented with 0.1 mg/l BA. The proembryos formed
friable embryogenic calli again after culture on medium supplemented with 10 mg/l picloram. The total time needed to regenerate
a complete plantlet from friable callus was approximately 6 months. This system for the production of embryogenic material
is considered to have valuable applications for genetic transformation in Alstroemeria.
Received: 22 April 1999 / Revision received: 16 July 1999 · Accepted: 20 July 1999 |
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Keywords: | Friable embryogenic callus Regeneration Rhizome Alstroemeria |
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