| 降低PARP酶活性对外源基因稳定整合的影响 |
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| 引用本文： | 杨其伟,陈雅文,史国利,陈德风,张自立YANG Qi-Wei,CHEN Ya-Wen,SHI Guo-Li,CHEN De-Feng,ZHANG Zi-Li.降低PARP酶活性对外源基因稳定整合的影响[J].遗传,1997,19(3):1-4. |
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| 作者姓名： | 杨其伟 陈雅文 史国利 陈德风 张自立YANG Qi-Wei CHEN Ya-Wen SHI Guo-Li CHEN De-Feng ZHANG Zi-Li |
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| 作者单位： | 1.南开大学分子生物学研究所, 天津300071
2.南开大学生化及分子生物学系，天津 300071
1.Institute of Molecular Biology,Nankai University,Tianjin 300071
2.Department of Biochemistry and Molecular Biology,Nankai University,Tianjin 300071 |
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| 摘 要： | 通过使用 PARP酶的抑制剂研究了降低PARP酶的活性对外源基因转染效率及整合作用的影响, 结果表明,转染的外源基因的吸收及瞬时表达不依赖于PARP酶的活性,而外源基因的整合作用与PARP酶的活性有关。
Abstract:In this study,the effect of lowering PARP activity on the transfection of cultured cells with foreigh genes was evaluated.The results indicated that PARP enzyme involved in the stable integration of foreign genes into the host genome.However,inhibition of PARP activity exhibited no effect on both the uptake into the cells and the expression of the forging genes.
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| 关 键 词： | PARP enzyme 瞬时表达 Key words 整合作用 抑制剂 PARP酶 等位基因多态性 MHC DQA基因 GC含量 超显性选择 纯化选择 |
Effects of Lowering PARP Enzyme Activity on the Stable Integration of the Exogenous Gene |
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| Yang Qiwei,Chen Yawen,Shi Guoli,Chen Defeng.Effects of Lowering PARP Enzyme Activity on the Stable Integration of the Exogenous Gene[J].Hereditas,1997,19(3):1-4. |
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| Authors: | Yang Qiwei Chen Yawen Shi Guoli Chen Defeng |
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| Abstract: | In this study, the effect of lowering PARP activity on the transfection of cultured cells with foreign genes was evaluated. The results indicated that PARP enzyme involved in the stable integration of foreign genes into the host genome. However, inhibition of PARP activity exhibited no effect on both the uptake into the cells and the expression of the foreign genes. |
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| Keywords: | PARP enzyme Inhibitor Stable integration Transient expression |
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