Cryopreservation of wheat suspension culture and regenerable callus |
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| Authors: | Tony H H Chen Kutty K Kartha Lawrence V Gusta |
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| Institution: | (1) Plant Biotechnology Institute, National Research Council, S7N 0W9 Saskatoon, Saskatchewan, Canada;(2) Present address: Alberta Research Council, 11315-87th Avenue, T6G 2C2 Edmonton, Alberta;(3) Present address: Crop Development Centre, University of Saskatchewan, S7N 0W0 Saskatoon, Saskatchewan, Canada |
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| Abstract: | Wheat (Triticum aestivum L. cv. Norstar) suspension cultures and regenerable calli initiated from immature embryos can be cryopreserved in liquid nitrogen temperature (–196°C) by slow freezing (0.5°C/min) in the presence of a mixture of DMSO and sucrose or sorbitol. Cold hardening or ABA treatment before cryopreservation increased the freezing resistance and improved the survival of wheat suspension culture in liquid nitrogen. Callus culture, established from immature embryos, prefrozen in 5% DMSO and 0.5M sorbitol survived liquid nitrogen storage and resumed plant regeneration after thawing. The results confirm the feasibility of long term preservation of wheat embryo callus by cryopreservation and retention of plant regeneration ability.Abbreviations ABA
Abscisic acid
- 2,4-D
2,4-Dichlorophenoxyacetic acid
- DMSO
Dimethylsulfoxide
- LN
Liquid nitrogen
- TTC
2,3,5-triphenyltetrazolium chloride
NRCC No. 23850. |
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| Keywords: | wheat cryopreservation suspension callus plant regeneration |
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