ATP synthesis associated with the conversion of hexachlorocyclohexane related compounds |
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Authors: | N Ohisa N Kurihara M Nakajima |
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Institution: | (1) Department of Agricultural Chemistry, University of Osaka Prefecture, 591 Sakai, Osaka, Japan;(2) Radioisotope Research Center, Kyoto University, 606 Kyoto, Japan;(3) Department of Agricultural Chemistry, Kyoto University, 606 Kyoto, Japan |
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Abstract: | Clostridium rectum strain S-17 converts -1,2,3,4,5,6-hexachlorocyclohexane (HCH) related compounds to chlorobenzenes. The metabolites from -1,2,3,4,5,6-hexachlorocyclohexene and -1,3,4,5,6-pentachlorocyclohexene are identified as 1,2,4-trichlorobenzene and 1,4-dichlorobenzene, respectively. ATP synthesis, converting these chlorinated compounds, is observed in the cell suspension of C. rectum as indicated by luciferase-luciferin reaction and phosphorylation of 32P-labeled phosphate. These observation lead to the conclusion that HCH and related compounds serve as artificial electron acceptors of the Stickland reaction, and therefore, the reductive dechlorination is associated with ATP synthesis.Abbreviations HCH
-1,2,3,4,5,6-hexachlorocyclohexane
- HCCH
-1,2,3,4,5,6-hexachlorocyclohexene
- PCCH
-1,3,4,5,6-pentachlorocyclohexene
- TCCH
-3,4,5,6-tetrachlorocyclohexene
- 1,2,4-TCB
1,2,4-trichlorobenzene
- 1,4-DCB
1,4-dichlorobenzene
- MCB
monochlorobenzene
- DTT
1,4-dithiothreitol
- IAA
monoiodoacetic acid |
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Keywords: | Chlostridium rectum Chlorobenzene HCH conversion Stickland reaction ATP synthesis Dechlorination |
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