| LALBA基因SNP与内蒙古白绒山羊经济性状的关联 |
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| 引用本文： | 蓝贤勇,陈宏,田智泉,刘少卿,张永斌,王昕,房兴堂.LALBA基因SNP与内蒙古白绒山羊经济性状的关联[J].遗传,2008,30(2):169-174. |
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| 作者姓名： | 蓝贤勇 陈宏 田智泉 刘少卿 张永斌 王昕 房兴堂 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100;2. 徐州师范大学细胞与分子生物学研究所, 徐州221166;3. 扬州大学动物科学与技术学院, 扬州 225009;4. 内蒙古鄂托克旗内蒙古阿尔巴白绒山羊种羊场 (三北羊场), 鄂尔多斯 016100 ; |
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| 基金项目： | 国家高技术研究发展计划(863计划)，西北农林科技大学校科研和教改项目，西北农林科技大学博士启动基金(西北农林科技大学人才基金)，陕西省自然科学基金，西北农林科技大学青年教师骨干项目 |
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| 摘 要： | 利用PCR-SSCP和DNA测序技术检测452份内蒙古白绒山羊α-乳白蛋白(LALBA)基因单核苷酸多态性(SNP), 并分析SNP与产绒量、绒厚、绒长和体重性状的关联。结果表明, 仅P2引物位点存在SSCP多态, 其外显子3区域存在1个突变位点: M63868:g.1897T>C。内蒙古白绒山羊群体LALBA基因M63868:g.1897位点以TT型为主, T等位基因频率为0.983, 且处于Hardy-Weinberg平衡状态(P>0.05)。方差分析表明, LALBA基因M63868:g.1897位点多态仅与产绒量存在显著相关(P=0.017); 1897位点TC基因型个体产绒量比TT基因型个体多产绒142.68 g, 高26.21%, 且差异显著(P<0.05)。因此, TC基因型可作为山羊产绒性状标记辅助选择的有效DNA标记。
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| 关 键 词： | 经济性状 关联 单核苷酸多态性(SNP) α-乳白蛋白 (LALBA) 基因 山羊 |
| 收稿时间： | 2007-08-07 |
| 修稿时间： | 2007年8月7日 |
Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat |
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| LAN Xian-Yong,CHEN Hong,TIAN Zhi-Quan,LIU Shao-Qing,ZHANG Yong-Bin,WANG Xin,FANG Xing-Tang.Correlations between SNP of LALBA gene and economic traits in Inner Mongolian white cashmere goat[J].Hereditas,2008,30(2):169-174. |
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| Authors: | LAN Xian-Yong CHEN Hong TIAN Zhi-Quan LIU Shao-Qing ZHANG Yong-Bin WANG Xin FANG Xing-Tang |
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| Institution: | 1. College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; 2. Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, Xuzhou 221116, China; 3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 4. Inner Mongolia White Cashmere Goat Breeding Farm, E’erduosi 016100, China |
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| Abstract: | PCR-SSCP and DNA sequencing methods were conducted to detect single nucleotide polymorphism of alpha-lactalbumin (LALBA) gene in 452 Inner Mongolian white cashmere goats (IMWC). Correlations between SNP of goat LALBA gene and economic traits, e.g., cashmere yield, cashmere thickness, length and weight, were analyzed. The SSCP in P2 primer locus, which was caused by the point mutation M63868:g.1897T>C in the exon 3 of LALBA gene was detected. At this locus, the genotype TT and allele T were predominant in the IMWC population, which agreed with Hardy-Weinberg equilibrium. Moreover, there was a significant correlation between polymorphism of goat M63868:g.1897 locus and cashmere yield of IMWC (P=0.017). The individuals with genotype TC had more cashmere yield than those with geontype TT. Hence, genotype TC of LALBA gene can be used as a molecular marker for breeding superior cashmere yield in goat marker-assisted selection. |
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