| 猪十二指肠组织灌流培养与静态培养比较 |
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| 引用本文： | 王绿阳,康翠翠,冯江银,丁立人,杭苏琴. 猪十二指肠组织灌流培养与静态培养比较[J]. 生物技术通报, 2021, 0(2): 246-252 |
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| 作者姓名： | 王绿阳 康翠翠 冯江银 丁立人 杭苏琴 |
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| 作者单位： | 南京农业大学国家动物消化道营养国际联合研究中心;南京农业大学动物科学类国家级实验教学示范中心 |
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| 基金项目： | 国家重点基础研究发展计划(973项目)(2013CB127301)。 |
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| 摘 要： | 组织灌流培养和静态培养是肠道组织体外培养最常见的两种方法,但目前尚不清楚这两种培养方法哪种更适合用于体外胃肠激素分泌的研究.以猪十二指肠组织为研究对象,首先比较L-精氨酸下两种体外培养方法对组织胆囊收缩素(Cholecystokinin,CCK)分泌和组织活性乳酸脱氢酶(Lactate dehydrogenase,LD...
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| 关 键 词： | 组织灌流培养 组织静态培养 L-精氨酸 胆囊收缩素 乳酸脱氢酶 |
Comparative Study of Pig Duodenum Between Tissue Perfusion Culture and Static Culture |
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| WANG Lü-yang,KANG Cui-cui,FENG Jiang-yin,DING Li-ren,HANG Su-qin. Comparative Study of Pig Duodenum Between Tissue Perfusion Culture and Static Culture[J]. Biotechnology Bulletin, 2021, 0(2): 246-252 |
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| Authors: | WANG Lü-yang KANG Cui-cui FENG Jiang-yin DING Li-ren HANG Su-qin |
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| Affiliation: | (National Center for International Research on Animal Gut Nutrition/Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health/Laboratory of Gastrointestinal Microbiology,Nanjing Agricultural University,Nanjing 210095;National Experimental Teaching Demonstration Center of Animal Science,Nanjing Agricultural University,Nanjing 210095) |
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| Abstract: | Tissue perfusion culture and static culture are the two most common methods of intestinal tissue culture in vitro,however,it is unclear which method is more suitable for the study of gut hormone secretion.This research used pig duodenum as the research object and chose L-arginine as stimulus.Firstly,we compared the effects of the 2 in vitro methods on cholecystokinin and lactate dehydrogenase secretion stimulated with L-arginine;furthermore,we made a comparison of the CCK levels in the duodenum treated with L-arginine in vivo,aiming to determine which method was more suitable for the study of gut hormone secretion in vitro.Results showed that CCK concentration fluctuated steadily and LDH concentration decreased gradually with the increase of time in the tissue perfusion culture;while in static culture,CCK and LDH concentration increased over time.In addition,the secretion of CCK in the tissue perfusion culture was very similar to that in blood.Above results demonstrate that the tissue perfusion culture is more suitable for the study of gut hormone secretion in vitro. |
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| Keywords: | tissue perfusion culture tissue static culture L-arginine CCK LDH |
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