体外模拟鳞杯伞子实体碱溶性多糖的消化与酵解特性

以热水浸提后的鳞杯伞Clitocybe squamulosa子实体残渣为原料,进行二次利用,浸提碱溶性多糖。通过模拟体外消化与厌氧发酵实验,探究鳞杯伞子实体碱溶性多糖的消化特性以及对肠道内短链脂肪酸含量的影响。结果表明：体外模拟唾液和胃肠液消化后,多糖的官能团结构特征没有发生显著性改变,但碱溶性多糖的块状结构解体,碎片化形态出现,分子量降低从(14.04±0.21) kDa到(10.48±0.26) kDa],还原糖含量升高从(0.06±0.01) mmol/L到(0.92±0.01) mmol/L]。在体外厌氧发酵条件下,发酵液中的OD₆₀₀显著性增加(P<0.05),而总糖含量和pH显著性降低(P<0.05);短链脂肪酸(SCFAs)总量从(1.40±0.06) mmol/L升高至(7.90±0.16) mmol/L。表明鳞杯伞碱溶性多糖可以被人体肠道菌群有效吸收利用,可以作为促进肠道健康的功能性食品进行研发。

Digestion and fermentation characteristics of alkaloid polysaccharide from Clitocybe squamulosa fruiting body in vitro

The residue of fruiting body of Clitocybe squamulosa after extraction by hot water was used to extract alkali soluble polysaccharides. Digestion and anaerobic fermentation experiments under in vitro simulated conditions were carried out to explore the digestion characteristics of the alkali soluble polysaccharides and the effect of the polysaccharides on the content of short chain fatty acids in intestinal tract. The results showed that there was no significant change in the functional group structure of polysaccharides after digestion by simulating saliva and gastrointestinal digestive juice in vitro; however, the bulk structure of the alkali soluble polysaccharide disintegrated, the molecular weight decreased from (14.04±0.21) to (10.48±0.26) kDa], and reducing sugar content increased from (0.06±0.01) to (0.92±0.01) mmol/L]. Under the condition of anaerobic fermentation in vitro, OD₆₀₀ in fermentation broth increased significantly (P<0.05), while total sugar content and pH decreased significantly (P<0.05). The total amount of short chain fatty acids (SCFAs) increased from (1.40±0.06) to (7.90±0.16) mmol/L]. The results show that the alkali soluble polysaccharides can be effectively absorbed and utilized by human intestinal microflora, and can be used as functional food to promote intestinal health.