汞化合物对红细胞膜作用的研究

本文研究了汞化合物对红细胞膜作用的光谱变化,观察了膜蛋白的荧光和磷光,膜上DPH的荧光偏振和ANS与红细胞膜的结合,以及他们与汞产生红血球溶血的关系。 在5P7.5缓冲液中红细胞膜蛋白的荧光随HgCl_2 Hg(AC)_2和PCMB的浓度加大而降低,表现为快和慢双相变化的过程,其淬灭作用的大小为HgCl_2>Hg(AC)_2PCMB,这是由于膜上形成了不发荧光的R—Trp—Hg~ 络合物以及能量从Trp转移到R—S—Hg~ 络合物上。HgCl_2对膜蛋白磷光的作用也是随汞离子浓度加大而降低,但磷光/荧光比则是增加的。标记红细胞膜的DPH偏振度是随HgCl_2浓度增加,表明膜流动性是随汞离子浓度加大而降低。标记膜上的ANS的荧光强度随HgCl_2和Hg(AC)_2的浓度加大而增加,这是由于ANS与膜的结合数随汞离子浓度加大而增加的缘故。上述各种变化是与汞离子对红血球溶血的作用一致的。

THE STUDIES ON THE RED BLOOD CELL MEMBRANE AFFECTED BY MERCURIDES

This paper reports the effects of mercurides on the red blood cell membrane including the changes of fluorescence and phosphorescence spectra of membrane protein; the fluorescence polarization of DPH labelled on membrane; the bindings of membrane with ANS and their relationships with the hemolysis of RBC affected by mercurides.The intensity of fluorescence of the membrane protein decreased with the increase of the concentration of mercurides. The quenching effect of the mercurides in 5p7.5 buffer solution is HgCl2> Hg(AC)2 PCMB. The quenching effect is two phases process in both 5p7.5 and PBS buffer solutions, but the slow phase of the quenching curve in PBS buffer solution changes very little. The fluorescence quenching effect of mercurides is due to the formation of non-fluorescent complex of R-Trp-Hg+ and the energy transfer from the Trp to the complex of R-S-Hg+. The intensity of phosphorescence of the membrane protein also decreased with the increase of the concentrtaion of HgCl2, but the ratio of P/F increased. As the concentration of HgCl2 and PCMB increase, the fluorescence polarization of DPH labelled on the ghost increased. The increase of polarization means the decrease of membrane fluidity which it may cause the hemolysis of the red blood cells. The dindings of ANS on the ghost protein increased with increase of mercurides, this result also coincides with the hemolvsis of RBC.