DNA ploidy pattern and amplification of ERBB and ERBB2 genes in human gastric carcinomas |
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Authors: | Noriko Oda Tetsuhiro Tsujino Toshitaka Tsuda Kazuhiro Yoshida Hirofumi Nakayama Wataru Yasui Eiichi Tahara |
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Institution: | 1. First Department of Pathology, Hiroshima University School of Medicine, 1-2-3 Kasumi, 734, Minami-ku, Hiroshima, Japan
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Abstract: | The DNA ploidy pattern and amplification of ERBB and ERBB2 genes were examined in paraffinembedded tissue from gastric carcinomas
using flow cytometry and a slot-blot hybridization technique. The incidence of aneuploidy in well differentiated adenocarcinomas
(56%) was significantly higher (p<0.05) than that in poorly differentiated adenocarcinomas (21%). The DNA ploidy pattern was not remarkably different between the
primary tumors and metastatic deposits in lymph nodes. Of the nine specimens having an aneuploid stem cell line in the primary
tumor and/or in metastases, three showed ERBB2 gene amplification and one showed ERBB gene amplification. The incidence of
epidermal growth factor (EGF) immunoreactivity in tumor cells showed no difference between diploid and aneuploid tumors. These
findings indicate that aneuploidy is frequently associated with amplification of ERBB and ERBB2 genes. |
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Keywords: | Gastric carcinoma DNA ploidy pattern ERBB ERBB2 |
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