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A tissue culture of the Ostrich fern Matteuccia struthiopteris (L.) Todaro
Authors:Gary Hicks  Patrick Von Aderkas
Institution:(1) Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada;(2) Atlantic Research Laboratory, National Research Council, 1411 Oxford Street, Halifax, Nova Scotia, Canada
Abstract:Multiple bud formation was induced from shoot apices of Matteuccia struthiopteris cultured on semi-solid Knudson's medium supplemented with 10-5 and 10-6 M kinetin. The effect of kinetin, naphthaleneacetic acid and gibberellic acid on shoot and root development is discussed and a three-part tissue culture system was devised for micropropagation and rooting.
Keywords:Matteuccia  micropropagation  kinetin  fern shoot tip culture
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