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Recent advances in engineering propionyl-CoA metabolism for microbial production of value-added chemicals and biofuels |
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| Authors: | Mark Bruder Lamees Akawi Dragan Miscevic Shane Kilpatrick Murray Moo-Young | |
| Affiliation: | Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada | |
| Abstract: | Diminishing fossil fuel reserves and mounting environmental concerns associated with petrochemical manufacturing practices have generated significant interests in developing whole-cell biocatalytic systems for the production of value-added chemicals and biofuels. Although acetyl-CoA is a common natural biogenic precursor for the biosynthesis of numerous metabolites, propionyl-CoA is unpopular and non-native to most organisms. Nevertheless, with its C3-acyl moiety as a discrete building block, propionyl-CoA can serve as another key biogenic precursor to several biological products of industrial importance. As a result, engineering propionyl-CoA metabolism, particularly in genetically tractable hosts with the use of inexpensive feedstocks, has paved an avenue for novel biomanufacturing. Herein, we present a systematic review on manipulation of propionyl-CoA metabolism as well as relevant genetic and metabolic engineering strategies for microbial production of value-added chemicals and biofuels, including odd-chain alcohols and organic acids, bio(co)polymers and polyketides.
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| Keywords: | Biofuels biopolymers metabolic engineering polyketides propionyl-CoA synthetic biology value-added products | |
