Enhancement of centelloside production from cultured plants of Centella asiatica by combination of thidiazuron and methyl jasmonate |
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| Authors: | Nam Hee Yoo Ok Tae Kim Jung Bong Kim Sun Hee Kim Young Chang Kim Kyong Hwan Bang Dong Yun Hyun Seon Woo Cha Min Young Kim Baik Hwang |
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| Affiliation: | (1) Industrial Cooperation Foundation, Chonbuk National University, Jeonju, 561-756, South Korea;(2) Planning and Coordination Division, National Institute of Horticultural and Herbal Science, RDA, Suwon, 440-706, South Korea;(3) Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 369-871, South Korea;(4) Functional Food and Nutrition Division, National Academy of Agricultural Science, RDA, Suwon, 441-853, South Korea;(5) Jeonnam Agricultural Research and Extension Service, Naju, 520-715, South Korea;(6) Department of Biology, Chonnam National University, Gwangju, 500-757, South Korea |
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| Abstract: | In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of madecassoside and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation. |
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