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Complex formation between metarhodopsin II and GTP-binding protein in bovine photoreceptor membranes leads to a shift of the photoproduct equilibrium |
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| Authors: | D Emeis H Kühn J Reichert K P Hofmann |
| Institution: | 1. Department of Biochemistry, Bowman Gray School of Medicine, Winston-Salem, NC 27103, USA;2. Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, NC 27103, USA;3. Department of Medicinal Chemistry, School of Pharmacy, University of North Carolina, Chapel Hill, NC 27514, USA |
| Abstract: | Cap binding protein (CBP)-related polypeptides were identified in different cytoplasmic RNP particles of embryonic chick muscles using monoclonal antibody to purified CBP. A single immunoreactive peptide (Mr 78000) was present in preparations of both free mRNP particles and a novel 10 S translation inhibitory RNP particle. In contrast, proteins isolated from these particles showed two new low-Mr immunoreactive peptides (Mr 43000 and Mr 29000). No CBP related protein could be detected in polysomal mRNP, although an immunoreactive Mr 43000 CBP-related protein was present in polysomes. The relevance of the association of different CBP-related polypeptides with cytoplasmic RNP particles and polysomes are discussed. |
| Keywords: | ROS rod outer segments G-protein GTP-binding protein MI metarhodopsin I MII metarhodopsin II GTP-γS NIR near-infrared |
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