Saikosaponin accumulation and antioxidative protection in drought-stressed Bupleurum chinense DC. plants |
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| Authors: | Zaibiao Zhu Zongsuo Liang Ruilian Han |
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| Institution: | 1. Research Center of Soil and Water Conservation and Ecological Environment, Chinese Academy of Sciences and Ministry of Education, No. 26 Xinong Road, Yangling, Shaanxi Province 712100, PR China;2. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China;3. Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, 8415683111, Isfahan, Iran;2. Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, 8415683111, Iran;3. Department of Pharmaceutical Biology and Botanical Garden of Medicinal Plants, Wroclaw Medical University, Wroc?aw, Poland;1. Department of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy;2. Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135, Torino, TO, Italy;3. CREA-VE, Viticulture and Enology Research Centre, Viale XXVIII Aprile 26, 31015, Conegliano, TV, Italy;4. Department of Agriculture, Food, and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy;5. Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126, Pisa, Italy;1. Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben 06466, Germany;2. Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India;3. Radiation Technology Development Section, Radio-Chemistry & Isotope Group, BARC, Mumbai 400 085, India;1. Technische Universität Braunschweig, Institute for Plant Biology, Mendelssohnstr. 4, 38106, Braunschweig, Germany;2. Agriculture Genetic Engineering Research Institute, AGERI, ARC, Giza, Egypt;1. Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran;2. Department of Agriculture, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran;1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, People’s Republic of China;2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People’s Republic of China |
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| Abstract: | Dried root of Bupleurum spp. is one of the most popular ingredients in many oriental medicinal preparations. Potted Bupleurum chinense DC. seedlings were subjected to progressive drought stress by withholding irrigation followed by a rewatering phase. The changes in antioxidant system, hydrogen peroxide (H2O2), superoxide radicals (O2?), and malondialdehyde (MDA) contents as well as saikosaponin a (SSa) and saikosaponin d (SSd) content in B. chinense roots were investigated. Additionally, the antioxidant activity of the roots extract was evaluated. The results showed that B. chinense root appeared highly resistant to water deficit. Both SSa and SSd content increased with the progressive water deficit, however, decreased under severe drought conditions or after water recovery. Moderate drought treatment resulted in 83% increase in SSa content and 22% increase in SSd content compared to the well-hydrated treatment. And increased SSa and SSd content during drought were accompanied by enhanced O2? content and superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) activity until severe drought stress. Notably, in vitra antioxidant tests demonstrated that the lipid peroxidation inhibition capacity was positively correlated with the content of SSa and SSd, particularly significant at p = 0.05 with SSd content. These results suggest that B. chinense roots exhibit effective antioxidative protection mechanism to withstand drought stress. And it could be speculated that drought-induced SSa and SSd accumulation in B. chinense roots may be stimulated via active oxygen species, and consequently involve in mitigating the oxidative damage due to its high anti-lipid peroxidation capacity. |
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