Impact of in vitro CO2 enrichment and sugar deprivation on acclimatory responses of Phalaenopsis plantlets to ex vitro conditions |
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| Authors: | Yeo-Joong Yoon Mohammad Mobin Eun-Joo Hahn Kee-Yoeup Paek |
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| Institution: | 1. Department of Horticulture, College of Life and Environmental Science, Daegu University, Gyungsan, Gyeonbuk, 38453, Republic of Korea;2. School of Agriculture, Meiji University, Kawasaki, Kanagawa, 214-8571, Japan;1. Institute of Natural Resources and Agrobiology of Salamanca, IRNASA-CSIC, Cordel de Merinas 40, E-37008 Salamanca, Spain;2. IRTA (Institute for Food and Agricultural Research and Technology), Field Crops, Av. Alcalde Rovira i Roure, 191, E-25198 Lérida, Spain;1. Department of Biology, East China Normal University, Shanghai 200241, China;2. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;1. Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA;2. Institute of Maize Research, Key Laboratory of Biology and Genetic Improvement of Maize in the Southwest Region, Ministry of Agriculture, Sichuan Agricultural University, Chengdu 611130, China;3. Department of Biological Sciences, Lehman College, City University of New York, Bronx, NY 10468, USA |
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| Abstract: | We assessed the effect of growth at either 400 μmol mol?1 (ambient) or 1000 μmol mol?1 (elevated) CO2 and 0 g L?1 (deprivation) or 30 g L?1 (supplementation) sugar on morphological traits, photosynthetic attributes and intrinsic elements of the CAM pathway using the CAM orchid Phalaenopsis ‘Amaglade’. The growth of shoot (retarded) and root (induced) was differently affected by CO2 enrichment and mixotrophic regime (+sugar). The Fv/Fm ratio was 14% more in CO2-enriched treatment than at ambient level during in vitro growth. At elevated level of CO2 and sugar treatment, the content of Chl(a + b), Chl a/b and Chl/Car was enhanced while carotenoid content remained unaltered. During in vitro growth, gas-exchange analysis indicated that increased uptake of CO2 accorded with the increased rate of transpiration and unchanged stomatal conductance at elevated level of CO2 under both photo- and mixotrophic growth condition. At elevated level of CO2 and sugar deprivation, activities of Rubisco (26.4%) and PEPC (74.5%) was up-regulated. Among metabolites, the content of sucrose and starch was always higher under CO2 enrichment during both in vitro and ex vitro growth. Our results indicate that plantlets grown under CO2 enrichment developed completely viable photosynthetic apparatus ready to be efficiently transferred to ex vitro condition that has far-reaching implications in micropropagation of Phalaenopsis. |
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