Target molecules of molecular chaperone (HSP70 family) in injured gastric mucosa in vivo |
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Authors: | Michiro Otaka Masaru Odashima Yuko Izumi Akihito Nagahara Taro Osada Naoto Sakamoto Makiko Takada Taiji Takahashi Yuji Shimada Kumiko Tamaki Daisuke Asaoka Hideaki Itoh Sumio Watanabe |
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Institution: | 1. Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1, Hongo Bunkyo-Ku, Tokyo, Japan;2. Department of Gastroenterology, Yokote City General Hospital, Negishi-Cho, Yokote City, Akita, 010, Japan;3. Department of Material-process Engineering and Applied Chemistry for Environment, Akita University Faculty of Engineering and Resource Science, Akita, Japan |
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Abstract: | AimsSeveral recent studies, including ours, have indicated the importance of heat shock proteins (HSPs) in cytoprotection against cytotoxic agents and environmental stresses mediated by the chaperone function of HSPs (molecular chaperones). However, the target molecule that is recognized by HSPs in damaged cells currently remains unknown. As HSPs rapidly recognize and bind to degenerated protein in cells, target molecules of HSPs might be key molecules for the initiation and pathogenesis of cellular damage. In the present study, gastric mucosal proteins that specifically bind to the HSP70 family (HSC70) were analyzed using HSC70-affinity chromatography.Main methodsThe gastric mucosa was removed from Sprague–Dawley rats after exposure to water immersion-stress for 0, 1, 3 or 5 h. Soluble fractions of each gastric mucosa were applied to the HSC70-affinity column separately. After washing off non-specific binding proteins, specific binding proteins were eluted by ATP-containing buffer. Binding proteins were analyzed by SDS-polyacrylamide gel electrophoresis. In addition, the amino acid sequence of purified proteins was also analyzed.Key findingsSpecific HSC70-binding proteins with a molecular weight of 200-kDa and 45-kDa were eluted from an affinity column when gastric mucosal homogenate of 1-h stress exposure was applied. The amino acid sequencing showed that these binding proteins were cytoskeletal myosin (heavy chain) and actin, respectively.SignificanceDuring the pathogenesis of stress-induced gastric mucosal damage, structurally degenerated cytoskeletal myosin (heavy chain) and actin may be key or initiation molecules which structural changes were firstly recognized by molecular chaperone. |
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