Morphological study of bacteria of the respiratory system using fluorescence microscopy of Papanicolaou-stained smears with special regard to the identification of Mycobacteria sp. |
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Authors: | TH KÜPPER U STEFFEN K WEHLE G RICHARTZ P PFITZER |
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Institution: | Institute of Cytopathology, Heinrich-Heine-University, Düsseldorf, Germany;Pneumologische Klinik, Ballenstedt, Germany |
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Abstract: | In Papanicolaou-stained smears certain structures such as nucleoli, Pneumocystis carinii , Charcot-Leyden crystals, bacteria and fungi show a brilliant fluorescence. the morphological characteristics of microorganisms which can be detected by this system, especially mycobacteria, are described. This screening method offers the possibility of providing the clinician with a provisional diagnosis within hours. Proof of the nature of the organisms should be obtained by culture. |
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Keywords: | mycobacteria tuberculosis Papanicolaou staining auramine staining fluorescence microscopy legionellae |
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