| SA脂质体介导DNA转染细胞的进一步研究 |
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| 引用本文： | 杨静平,孔玉英.SA脂质体介导DNA转染细胞的进一步研究[J].生物化学与生物物理学报,1994,26(2):123-128. |
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| 作者姓名： | 杨静平 孔玉英 |
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| 作者单位： | 中国科学院上海生物化学研究所分子生物学国家重点实验室，中国科学院上海生物化学研究所真核基因表达调控实验室 |
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| 摘 要： | ＳＡ脂质体可高效介导ＤＮＡ转染ＣＶ－１细胞，本文进步研究表明，ＳＡ脂质体还可介导ＤＮＡ高效瞬时和稳定地转染ＣＨＯ和ＣＯＳ细胞。ＳＡ脂质体和ＤＮＡ形成复合物可保护ＤＡＮ不被核酸内切酶和ＤＮａｓｅＩ降解。荧光标记和细胞松驰素Ｂ抑制实验分别表明，ＳＡ脂质体易被细胞吸附，主要通过内吞传送ＤＮＡ进入细胞，而Ｌｉｐｏｆｅｃｔｉｎ主要通过融合传送ＤＮＡ进细胞。
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| 关 键 词： | SA脂质体 基因 转染 机理 |
Further Studies on SA Liposome Mediated Transfection |
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| YANG Jing-Ping, KONG Yu-,Ying, and LIN Qi-Shui.Further Studies on SA Liposome Mediated Transfection[J].Acta Biochimica et Biophysica Sinica,1994,26(2):123-128. |
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| Authors: | YANG Jing-Ping KONG Yu- Ying and LIN Qi-Shui |
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| Abstract: | SA liposomes can effectively mediate DNA transfection of OV-1 cells as well as the transient and stable DNA transfection of OHO and OOS cells. SA liposomeDNA complexes can Protect DNA from digestion by restriction enzymes and DNase I. Results of fluorescence labeling and cytochalasin B inhibiting experiments indicated that SA liposomes could be easily absorbed to the surface of cells and DNA was delivered into oelle mainly through endocytosis, but lipofectin delivered DNA into cells mainly by fusion. |
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| Keywords: | SA liposome Gene Transfection Mechanism |
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