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| 链霉菌702产孢子固体培养基和培养条件的筛选 | |
| 引用本文： | 熊智强,徐平,武琳,张嗣良,涂国全.链霉菌702产孢子固体培养基和培养条件的筛选[J].生物数学学报,2008,23(3):563-568. |
| 作者姓名： | 熊智强 徐平 武琳 张嗣良 涂国全 |
| 作者单位： | 1. 华东理工大学,生物反应器国家重点实验室,国家生化工程技术研究中心,上海,上海,200237;江西农业大学,生物科学与工程学院,江西,南昌,330045 2. 江西农业大学,生物科学与工程学院,江西,南昌,330045 3. 华东理工大学,生物反应器国家重点实验室,国家生化工程技术研究中心,上海,上海,200237 |
| 基金项目： | 国家教育部重点项目，江西省自然科学基金 |
| 摘 要： | 通过单因素实验、均匀设计和正交实验对链霉菌702产抱子培养基和培养条件进行筛选，筛选到的最佳培养基组成为：马铃薯2009／L，葡萄糖25g／L．最佳培养条件为培养温度37℃、培养时间5天和培养基初始pH值8．实验结果表明，采用优化后的培养基和培养条件，使链霉菌702产抱子数达到4．07亿，比原来培养基（高氏一号培养基）产抱予量提高了8．7倍，培养时间却从原来的7天缩短到现在的5天． |
| 关 键 词： | 链霉菌702 孢子 培养 均匀设计 |
Screening of Solid Medium and Cultural Conditions on Producing Spores for Streptomyces 702 |
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| XIONG Zhi-qiang,XU Ping,WU Lin,ZHANG Si-liang,TU Guo-quan.Screening of Solid Medium and Cultural Conditions on Producing Spores for Streptomyces 702[J].Journal of Biomathematics,2008,23(3):563-568. | |
| Authors: | XIONG Zhi-qiang XU Ping WU Lin ZHANG Si-liang TU Guo-quan |
| Institution: | XIONG Zhi-qiang, XU Ping, WU Lin, ZHANG Si-liang, TU Guo-quan (1 State Key Laboratory of Bioreaetor Engineering, East China University of Science and Technology, National Engineerin9 Research Center of Bioteehnology(shanghai), Shanghai 200237 China) (2College of Life Sciences and technology, Jiangxi Agricultural University, Nanchang Jiangxi 330045 China) |
| Abstract: | The optimum medium and cultural conditions on producing spores for Streptomyces 702 were screened out through uniform design, orthogonal and single factor experiment. The optimum medium consisted of （g/L）： potato 200, glucose 25. The optimal conditions were 37℃ of cultural temperature, 8 of initial pH, 5d of cultural time. The experimental results showed that, under the optimal conditions, the number of spores produced by Streptomyces 702 was up to 407 millions, which was increased by 870 % than the original medium （Gause＇s No.1 synthetic medium）. Furthermore, the culture time was shortened from 7d to 5d. |
| Keywords: | Streptomyces 702 Spore Culture Uniform design |
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